DNR (Deoxyribonucleic Acid) and RNA (Ribonucleic Acid) oligonucleotides are short sequences of nucleotides that can be synthesized for various biological and medical applications.
DNA Oligonucleotides
DNA oligonucleotides are short single- or double-stranded DNA molecules. They are commonly used in:
- PCR (Polymerase Chain Reaction): Amplifying DNA sequences for various experiments.
- Gene Synthesis: Creating specific sequences for cloning, gene editing, or studying gene function.
- Hybridization Probes: Used in assays like Southern blotting to detect specific DNA sequences.
- Antisense Oligos: Designed to bind to a specific mRNA, blocking translation and modulating gene expression.
RNA Oligonucleotides
RNA oligonucleotides are typically single-stranded and are used in various applications:
- RNA Interference (RNAi): Small interfering RNAs (siRNAs) or microRNAs (miRNAs) to silence specific gene expressions.
- CRISPR-Cas9: Guide RNAs (gRNAs) used in gene editing.
- mRNA Research: Studying gene expression, RNA splicing, and mRNA translation.
- Therapeutic Applications: Developing mRNA-based vaccines and therapies.
Applications of Synthesized Oligonucleotides
- PCR primers and probes.
- siRNA and miRNA for gene silencing.
- ASO (Antisense Oligonucleotides) for therapeutic applications.
- CRISPR guide RNAs for gene editing.
- DNA/RNA aptamers for molecular recognition.
Hello world!
Welcome to WordPress. This is your first post. Edit or delete it, then start writing!