Applications of Synthesized Oligonucleotides
- PCR primers and probes.
- siRNA and miRNA for gene silencing.
- ASO (Antisense Oligonucleotides) for therapeutic applications.
- CRISPR guide RNAs for gene editing.
- DNA/RNA aptamers for molecular recognition.
DNR (Deoxyribonucleic Acid) and RNA (Ribonucleic Acid) oligonucleotides are short sequences of nucleotides that can be synthesized for various biological and medical applications.
DNA Oligonucleotides
DNA oligonucleotides are short single- or double-stranded DNA molecules. They are commonly used in:
- PCR (Polymerase Chain Reaction): Amplifying DNA sequences for various experiments.
- Gene Synthesis: Creating specific sequences for cloning, gene editing, or studying gene function.
- Hybridization Probes: Used in assays like Southern blotting to detect specific DNA sequences.
- Antisense Oligos: Designed to bind to a specific mRNA, blocking translation and modulating gene expression.
RNA Oligonucleotides
RNA oligonucleotides are typically single-stranded and are used in various applications:
- RNA Interference (RNAi): Small interfering RNAs (siRNAs) or microRNAs (miRNAs) to silence specific gene expressions.
- CRISPR-Cas9: Guide RNAs (gRNAs) used in gene editing.
- mRNA Research: Studying gene expression, RNA splicing, and mRNA translation.
- Therapeutic Applications: Developing mRNA-based vaccines and therapies.
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